Identification of gonadotrophin surge-inhibiting factor (GnSIF)/attenuin in human follicular fluid
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چکیده
منابع مشابه
Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid.
Gonadotrophin surge attenuating factor (GnSAF) is a new non-steroidal ovarian substance, different from inhibin, which attenuates the pre-ovulatory luteinizing hormone (LH) surge in superovulated women. Human follicular fluid (FF) was used as a source for the isolation of GnSAF, the activity of which was monitored in an in-vitro pituitary bioassay. Primary rat pituitary cells were incubated wit...
متن کاملSelection of gonadotrophin surge attenuating factor phage antibodies by bioassay
BACKGROUND We aimed to combine the generation of "artificial" antibodies with a rat pituitary bioassay as a new strategy to overcome 20 years of difficulties in the purification of gonadotrophin surge-attenuating factor (GnSAF). METHODS A synthetic single-chain antibody (Tomlinson J) phage display library was bio-panned with partially purified GnSAF produced by cultured human granulosa/luteal...
متن کاملEffect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples
Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosper...
متن کاملThe recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor.
BACKGROUND Gonadotrophin surge-attenuating factor (GnSAF) is an as yet unidentified ovarian factor that acts on the pituitary to attenuate the pre-ovulatory LH surge. In a previous study, GnSAF bioactivity was proposed to derive, at least in part, from a C-terminal domain (95peptide) of human serum albumin (HSA). METHODS AND RESULTS We employ here the expression-secretion system of Pichia pas...
متن کاملLeukaemia inhibitory factor expression in human follicular fluid.
Leukaemia inhibitory factor expression in human follicular fluid Dear Sir, We were interested to read the paper by Arici et al. (1997) who found leukaemia inhibitory factor (LIF) expression in human follicular fluid (hFF). We would like to confirm this fact and make some short comments. Since we have postulated that LIF could play a role in the inflammatory-like processes involved in the periov...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1996
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/11.3.490